Table 1 Challenges in aptamer biosensors
From: Aptamer technology for tracking cells’ status & function
Challenges | Possible solutions |
|---|---|
Strategies to enhance in vivo pharmacokinetics | - Formation of aptamer conjugates |
- Aptamer chemical modifications | |
Loss of in vivo affinity for in vitro selected aptamers | - Direct in vivo aptamer selection |
- Adjusting in vitro selection conditions to mimic closely in vivo conditions (e.g. pH, temp) | |
- Polyvalent aptamer conjugates | |
Cost of aptamer biosensors | - Minimizing aptamers length |
- Utilize DNA-based aptamer | |
Safety of aptamer biosensors | - Complete pre-clinical studies |
- Negative selection with closely related molecular targets |