Fig. 6
From: Interleukin 20 regulates dendritic cell migration and expression of co-stimulatory molecules
Adhesive properties of cytokine-treated MDDCs. Cells were incubated with IL-20 or TNFα prior to microscope imaging (a-f) or analysis of β2 (CD18) integrin adhesion molecules shedding (g,h). MDDCs were imaged either as untreated (a,b), following treatment with 0.01 ng/ml IL-20 (c,d), or with 100 ng/ml TNFα (e,f). g,h CD18 shedding from MDDCs treated with cytokines. Based on cells from seven donors (#60-#66), culture supernatants from cytokine-treated MDDCs were applied in analyses of the concentration of sCD18. Comparison of sCD18 concentration (in mU/ml) obtained from either untreated cultures, cultures stimulated with 0.01-5000 ng/ml of IL-20 (g), or 1-100 ng/ml of TNFα (h)