Fig. 4
From: Interleukin 20 regulates dendritic cell migration and expression of co-stimulatory molecules
p38 MAPK and STAT3 phosphorylation in MDDCs pretreated by cytokines from the IL-10 family. Phosphorylation of p38 was measured for Thr-180 and Tyr-182 and for Tyr-705 in STAT3. Data were based on cellular cultures derived from 3 donors and measured in duplicates. The data are shown as the mean values of (arbitrary) fluorescence units with error bars indicating the SD. Interleukin pretreatment was started 10 min before harvest with the cytokines added at concentrations (ρIL) of 0.001, 0.1, 1, or 10 ng/ml (Incubation V in Fig. 1b) and compared with cultures without cytokine addition. The influence was tested of IL-10 on p38 (a) and STAT3 (b) phosphorylation, and similarly for IL-19 (c,d), IL-20 (e,f), and IL-24 (g,h)