Fig. 2

CD86 expression of IL-20-treated MDDCs. Cells were treated according to Incubation III outlined in Fig. 1b with addition of IL-20 to the culture 4-6 h before harvest. IL-20 was applied in concentrations (ρIL-20) of 0.001-5000 ng/ml and cells from 3-11 donors were analyzed. Results were based on MDDCs from Donors #1-25. a The percentage of CD86 positive MDDCs following incubation without or with IL-20 in the concentration stated on the abscissa. Bars indicate the statistical significant difference between treated and non-treated MDDCs cultures as analyzed by a t-test (b-d). Flow cytometric determination of the level of staining with antibodies to CD40 and CD86. As a representative case, results from the analysis of Donor 1 is shown, either as unstimulated (b), stimulated with LPS (c), or stimulated with IL-20 (d). e Histogram showing the distribution of staining intensities with Ab to CD86, either for untreated culture or for cultures treated with IL-20