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Fig. 4 | Molecular and Cellular Therapies

Fig. 4

From: Proteomic analysis of extracellular vesicles from medullospheres reveals a role for iron in the cancer progression of medulloblastoma

Fig. 4

Iron chelation decreased DAOY Medulloblastoma sphere formation and growth. a Representative image of DAOY spheres at 5X and 10X magnification in the presence of iron chelators in the culture medium (100 μM deferoxamine (DFO), 1 μM Dp44mT) or of iron (50 μM Ferric ammonium citrate) or 1 μM Dp44mT + 50 μM FAC. b Count by light microscopy of number of spheres obtained in a T75 low adhesion plate after 72 h of culture in the presence of 100 μM DFO, 1 μM Dp44mT, 50 μM FAC or 1 μM Dp44mT + 50 μM FAC (*p < 0.05). c Area of the spheres in arbitrary units (A.U) obtained in a T75 low adhesion plate after 72 h of culture in the presence of 100 μM DFO, 1 μM Dp44mT, 50 μM FAC or 1 μM Dp44mT + 50 μM FAC (*p < 0.05). d Limited dilutions assay in which 1 cell was plated in each low adhesion culture 96 wells -plate and after 14 days, the number of clonal tumor spheres formed in each well were counted in the presence of 100 μM deferoxamine (DFO), 1 μM Dp44mT, 50 μM of Ferric ammonium citrate or 1 μM Dp44mT + 50 μM FAC (*p < 0.05)

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