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Fig. 2 | Molecular and Cellular Therapies

Fig. 2

From: Proteomic analysis of extracellular vesicles from medullospheres reveals a role for iron in the cancer progression of medulloblastoma

Fig. 2

Proteomic analysis of EVs from MB, MBS and gene ontology analysis. a EVs from DAOY, UW228 and ONS-76 MB (adhesion culture) and MBS (spheres) were separated on SDS-PAGE. Coomassie-G bands were excised from the gels and destained. In-gel enzymatic digestions were performed before mass spectrometry analysis. b Representative network of the proteins (nodes) experimentally identified in adherent cells (purple), spheres (green) or both (magenta) and already described together in at least one paper (edges). The node size is proportional to the number of papers in which the proteins have been studied, while the edge width is proportional to the document number in which two proteins have been described together (data obtained by means of ProteinQuest). c Western blot analysis of EVs extracted from MB (adhesion), MBS (spheres) of DAOY, UW228 and ONS76 cells. Antibodies against Hemopexin were used. Actin was used as a loading control

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