Figure 4

Paw fluorescence from arthritic and non-arthritic mice 24 h post-injection. IVIS Spectrum in vivo imaging of arthritic and non-arthritic mice 24 h post-injection of a 54 kDa dye-labeled copolymer (Pol-dye) and dye alone. Multispectral fluorescence images were unmixed to subtract background fluorescence enabling visualization of the dye and dye-labeled polymers. The images show representative examples of polymer accumulation in arthritic joints, minute amounts of polymer in joints of healthy mice, and no presence of dye alone as control that correspond to Figure 3.