Fluo-4 calcium mobilization assay in human NS/PCs derived neurons. (A-C) Fluo-4 calcium mobilization assay (data represent 3 independent experiments) in hNS/PCs derived neurons transduced with lentiviral vectors expressing Anti-miR-223 sponge or mismatched sponge as a control. (A) Fluo-4 fluorescence intensity before (upper panels) and after (lower panels) 100 μM NMDA stimulation. (B) Relative intensity time course of fluo-4 fluorescence corresponding to calcium influx following NMDA stimulation. (C) Relative fluo-4 intensity quantification of indicated time points (n = 7, *p < 0.05, Student’s t-test).