Figure 1

General upregulation of miRNAs following NS/PCs differentiation. (A) Immunocytochemistry for the undifferentiated and differentiated hNS/PCs. (B) Upper panel: Taqman low-density array (TLDA) analysis. Relative expression levels (heat-map) after global mean normalization are shown as log2 ratios for undifferentiated “U”, differentiated “D” and the fold change in differentiated relative to undifferentiated “D/U” hNS/PCs. TLDA was performed in 2 independent experiments. Lower panel: a plot demonstrating the fold change of individual miRNAs. Each square represents one miRNA. (C) Select tissue distribution comparison for human miR-223 predicted targets. (D) Gene ontology analysis for human brain-expressed miR-223 predicted targets. The left Y-axis represents the EASE score (Expression Analysis Systematic Explorer; a modified Fisher Exact p-Value) p-values for gene-enrichment annotation cluster terms. The right Y-axis represents the cluster enrichment scores.