Figure 6

Proliferation and differentiation rates. A. Proliferation rates of the ahiPSCs, in the embryoid bodies differentiating towards cardiomyocytes, for 12 days and 61 days, were determined by ratios of bromodeoxyuridine (BrdU) to cardiac myosin heavy chains (MHC) normalized against all cells. B. Progressing differentiation was determined by counting the increasing number of embryoid bodies initiated from the hESCs and ahiPSCs. C. Progressing differentiation associated with suppression of NANOG expression - the key pluripotency gene in the EBs initiated from the hESCs and the ahiPSCs after 1 day (hESC 1d and ahiPSC 1d) versus 60 days (hESC 60d and ahiPSC 60d), while compared to the undifferentiated hESCs and ahiPSCs (undif hESC and undif ahiPSC) as well as the PBMCs (undif PBMC 1d and undif 60d). The statistical significance was accepted for P < .05.