Figure 4

Electrophoresis of reverse transcribed and amplified ahiPSCs pluripotency genes’ transcripts. RNA was extracted from the ahiPSCs and PBMCs, reverse transcribed to cDNA, and amplified. A. Amplicons were electrophoresed and imaged. B. The lanes were quantified. Amplicons on the lanes: 1–4, 3–8, 9–12 for the patients C001-C003 respectively; 13–14, 16 standards; 15 no primers. The amplicons of ahiPSCs on the lanes: 1,3,5,7,9,11 and of PBMCs on the lanes: 2,4,6,8,10,12; OCT4: lanes 1–2, 5–6, 9–10, NANOG: lanes 3–4, 7–8, 11–12.