Figure 2

Molecular architecture of sarcomeres in infarcted myocardium. (A) Preservation of the myofibril structure was imaged by phase contrast light microscopy (Z = Z-line; A = A-band). Underneath the image, there is the corresponding, quantified analysis of the spatial distribution of the optical density (Y axis - relative units). It is a way to estimate sarcomers’ structural integrity. (B-E) Distribution of proteins was determined with the htAbs carrying chelated atoms of exogenous elements and emitting specific spectra. Colors were assigned artificially. Underneath the images, there are quantifications of spatial distribution of fluorescence intensity. Correlations between A and B-E determine, if the molecules retain their positioning within the sarcomeres’ architecture. HFW: 4.8 μm. Pixel brightness compared between the specific labels and the backgrounds during acquisition was accepted at the statistical significance P < .0001.