Figure 1

Primary CD4 T cells were in vitro infected with the PI-resistant AD.MDR01 and the PI-susceptible Donor E HIV-1 strains in the presence or absence of efavirenz, lopinavir or raltegravir. A) Cell viability over time as measured by trypan blue exclusion. B) HIV p24 concentration in cell culture supernatants was measured by ELISA at day 6 post infection. C) Apoptosis as measured by TUNEL staining and flow cytometry at day 6 post infection; representative results of three independent experiments. D) The percent of cells TUNEL positive at day 6 post infection, expressed as mean +/- standard error of three independent experiments, was compared by ANOVA. * indicates a significant P value <0.05 compared to untreated infected cells.