Figure 4

Recruitment and retention of haBMSCs to infarcted myocardia. (A) The isolated populations of cells with the cell surface display of CD34, CD117, CD133 (each tagged with a different elemental tag) were applied either as a single batch of haBMSCs mixed with clusters of differentiation saturating remaining binding sites (e.g., haBMSCs CD34+ with CD117, CD133) or as a mix of all three populations in equal ratios. They were compared to the assay in which the haBMSCs were applied without preceding htAbs (“C” in this figure). Applying all three native receptors blocked binding sites on the htAbs and prevented the haBMSCs from docking. The statistical significance was accepted at P = .0003. (B) Retention of the haBMSCs onto the sarcomeres was measured at different periods of time for samples maintained in the environmental incubator. The difference between the numbers of cells retained to the sarcomeres, when they were preceded by administration of the htAbs (htAb) was much higher than preceded by administration of plain buffer with no antibodies (no Ab). Once the haBMSCs anchored to the sarcomeres on the day one of the experiment (1d) , thereafter they were retained with minimal losses for up to 12 days studied (12d). The statistical significance was accepted at P = .0003. (C) Specificity of recruitment of the CD34+, CD117+, CD133+ haBMSCs’ populations was determined after mixing them in equal ratios and administration to myocardial infarction models, while preceded by injections of the htAbs (htAb), non-specific antibodies (nsAbs), or plain buffer without any antibodies (no Ab). In the assays, which included htAbs, the numbers of the cells attached were much higher, totaling more than 80%, in comparison with the assays including non-specific or excluding all antibodies. The statistically significant difference was accepted at P = .0003.