Figure 2

Preservation of architecture and antigenicity of myosin in infarcted myocardia. (A) The Zernicke’s phase-contrast microscopy showed the overall structure of the sarcomeres in the human, native, cardiac myofibrils from the central zone of infarcted myocardium. (B) Cardiac myosin (in the sarcomeres of the myofibrils from A) was labeled with the fluorescent htAbs against myosin and highlighted in multiphoton fluorescence spectroscopy. The anti-myosin htAbs overlap exactly the A-bands of the sarcomeres. (C) Gels of the four patients' (MI/HT001-004) cardiac tissues, which were disintegrated, electrophoresed, and stained, revealed prominent and sharp bands of myosin, actin, tropomyosin, troponin, actinin, and titin. These show preservation of molecular integrity of the sarcomeric proteins. (D) The same samples as those shown in C, were transferred onto PVDF membranes, and labeled with the htAbs against myosin. The only bands are exquisitely specific for the labeled myosin. These are also indications of preservation of myosin antigenicity. The entire lanes are shown to demonstrate very specific labeling and lack of any non-specific binding. Axial pixel brightness compared between specific signals and backgrounds was accepted at P = .0003.